26, rue d'Ulm 75248 Paris Cedex 05 Marina.Glukhova@curie.fr

S É M I N A I R E

 Alexander Chenchik

Cellecta, Inc., Mountain View, CA USA

Public Decipher Genetic Screen Platform: Discovery Drug Targets in Cancer Cell Models

Invité(e) par Marina Glukhova

Mercredi 23 Juin 2010
16 heures

We have developed a free open source Decipher genetic screen technology for the functional discovery of drug targets associated with viability and regulation of signaling pathways in cancer cells. The Decipher platform combines premade human and mouse pooled bar-coded 27K lentiviral shRNA libraries targeting set of 5,000 most critical regulators of all described 1,200 signaling pathways (modules), drug metabolism, transporters, drug resistance, disease-associated genes and FDA approved drug targets; supporting knowledge database and data analysis and annotation software tools. A pooled lentiviral shRNA library is used to transduce a cancer (reporter) cell line, and cells demonstrating modulation in a specific pathway or lost in viability are isolated by FACS, drug selection, growth selection, etc. shRNA effectors responsible for modulating cell signaling pathways are then identified by HT sequencing. Data will be presented that demonstrated application, problems, and solutions for genome-wide RNAi functional analysis with pooled effector libraries for several cancer cell models.

Institut Curie
26, rue d'Ulm -- 75005 Paris
Salle de conférence Antoine Lacassagne
Rez-de-chaussée du Pavillon Trouillet-Rossignol